Fusion of HSV-1 VP22 to a bifunctional chimeric SuperCD suicide gene compensates for low suicide gene transduction efficiencies

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Fusion of HSV-1 VP22 to a bifunctional chimeric SuperCD suicide gene compensates for low suicide gene transduction efficiencies.

Low transduction efficiencies of viral and non-viral vectors still remain a major limitation in suicide gene therapy. The HSV-1 tegument protein VP22 can spread from cells where it is produced to surrounding recipient cells, thus making it a promising tool for compensation of inadequate gene transfer efficiencies. In our previous study, we focused on the optimization of the cytosine deaminase (...

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Gene therapy has become a promising alternative treatment form for cancer. Among the broad range of different genetic means to reduce the tumor growth, herpes simplex virus thymidine kinase/ganciclovir (HSV-TK/GCV) suicide gene therapy regimen is the best known approach. In this type of therapy, cancer cells are manipulated to express HSV-TK, followed by administration of the prodrug, the antiv...

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The Herpes Simplex virus thymidine kinase gene (HSV-tk) has been widely used as a suicide gene in cancer gene therapy. Since the f irst demonstration of the abil ity of HSV-tk gene modified tumor cells to generate a bystander effect, a number of clinical trials have been initiated to treat human cancers. However, the mechanism of the HSV-tk mediated bystander tumor kil l ing has remained contro...

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ژورنال

عنوان ژورنال: International Journal of Oncology

سال: 2007

ISSN: 1019-6439,1791-2423

DOI: 10.3892/ijo.30.5.1153